Author/Authors :
Badali, Hamid Department of Medical Mycology -Invasive Fungi Research Center (IFRC) - School of Medicine - Mazandaran University of Medical Sciences, Sari, Iran , Rezaie, Sassan Department of Medical Mycology and Parasitology - Tehran University of Medical Sciences, Tehran, Iran , Meis, Jacques. F Department of Medical Microbiology and Infectious Diseases - Canisius-Wilhelmina Hospital, Nijmegen, Netherlands , Agha Kuchak Afshari, Setareh Department of Medical Mycology and Parasitology - Tehran University of Medical Sciences, Tehran, Iran , Modiri, Mona Department of Medical Mycology and Parasitology - Tehran University of Medical Sciences, Tehran, Iran , Hagen, Ferry Centre of Expertise in Mycology Radboudumc/CWZ - Department of Medical Microbiology Nijmegen, Netherlands , Moazeni, Maryam Department of Medical Mycology -Invasive Fungi Research Center (IFRC) - School of Medicine - Mazandaran University of Medical Sciences, Sari, Iran , Mohammadi, Rasoul Department of Medical Parasitology and Mycology - School of Medicine -Infectious Diseases and Tropical Medicine Research Center - Isfahan University of Medical Sciences, Isfahan, Iran , Khodavaisy, Sadegh Department of Medical Mycology and Parasitology - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background and Purpose: Candida parapsilosis is a predominant species found in nosocomial infection, particularly in hospitalized patients. The molecular epidemiology of the clinical strains of this species has not been well studied. The present study was performed with the aim of investigating the microsatellite genotyping of Candida parapsilosis among the Iranian clinical isolates.
Materials and Methods: This study was conducted on 81 independent clinical C. parapsilosis isolates that were genotyped by using a panel of six microsatellite markers.
Results: The short tandem repeat (STR) typing of clinical C. parapsilosis isolates demonstrated 68 separate genotypes, among which 57 genotypes were observed once and the remaining 11 cases were identified for multiple times. The Simpson’s diversity index for the panel of combined six markers yielded a diversity index of 0.9951. The heterogeneity was observed among the Iranian and the Netherlands clinical C. parapsilosis isolates.
Conclusion: As the findings indicated, the clinical C. parapsilosis isolates from Iran showed a high genetic diversity. It can be concluded that molecular epidemiology could be useful for screening during outbreak investigation where C. parapsilosis is involved.
Keywords :
Microsatellite , Iran , Candida parapsilosis , Genotyping
