Author/Authors :
Alizadeh, Majid Department of Parasitology and Mycology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Kolecka, Anna Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands , Boekhout, Teun Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands , Zarrinfar, Hossein Allergy Research Center - Mashhad University of Medical Sciences, Mashhad, Iran , Ghanbari Nahzag, Mohamad. A Department of Parasitology and Mycology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Badiee, Parisa Alborzi Clinical Microbiology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Rezaei-Matehkolaei, Ali Department of Medical Mycology - School of Medicine - Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Fata, Abdolmajid Department of Parasitology and Mycology - Research Center for Cutaneous Leishmaniasis - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Dolatabadi, Somayeh Faculty of Engineering - Sabzevar University of New Technologies, Sabzevar, Iran , Najafzadeh, Mohammad. J Cancer Molecular Pathology Research Center - Mashhad University of Medical Sciences, Mashhad, Iran
Abstract :
Background and Purpose: Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify Candida isolates by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) from women with vulvovaginitis that were referred to Ghaem Hospital, Mashhad, Iran.
Materials and Methods: This study was conducted on 65 clinical samples isolated from women that were referred to Ghaem Hospital. All specimens were identified using phenotyping techniques, such as microscopy and culture on Sabouraud dextrose agar and corn meal agar. In addition, all isolates were processed for MALDI-TOF MS identification.
Results: Out of the 65 analyzed isolates, 61 (94%) samples were recognized by MALDI-TOF MS. However, the remaining four isolates (6%) had no reliable identification. According to the results, C. albicans (58.5%) was the most frequently isolated species, followed by C. tropicalis (16.9%), C. glabrata (7.7%), C. parapsilosis (7.7%), and guilliermondii (3.1%).
Conclusion: As the findings indicated, MALDI TOF MS was successful in the identification of clinical Candida species. C. albicans was identified as the most common Candida species isolated from the women with VVC. Moreover, C. tropicalis was the most common species among the non-albicans Candida species.
Keywords :
Candidiasis , Identification , MALDI-TOF MS , Vulvovaginitis
