Time dependent difference effects of MgO and ZnO nanoparticles on the serum and hippocampus Mg^2+, Zn^2+, Fe^2+/3+ and Ca^2+ levels in the stressed rats

Objective(s): Stress is a physiological response that can disrupt body elements homeostasis and lead to neurophysiological abnormality. This study has been investigated the serum and hippocampus Mg^2+, Zn^2+, Fe^2+/3+ and Ca^2+ level changes in two times after MgO NPs and ZnO NPs single injection following restraint stress in the male rat. Methods: Animals were divided into two main groups that each of them includes: control, restraint of 90, 180 and 360 min+ saline, MgO NPs and ZnO NPs 5 mg/kg alone and with a restraint of 90 min. In one group, 30 min and in another 120 min after intraperitoneally injections of components or stress induction elements levels were measured in the serum/ hippocampus. Results: Elements level changed in the serum and hippocampus following injections of MgO and ZnO NPs depend on acute time after injections. ZnO NPs induced a positive correlation between serums Fe^2+/3+ levels in two acute times. Different times of stress induction have different effects on elements level changes in the serum and hippocampus, 30 and 120 min after induction and nanoparticles could alleviate these changes depend on the time. In restraint groups, there were positive and negative significant correlations between two different times measurements of Fe^2+/3+ or Ca^2+ in the serum and hippocampus. Conclusion: it seems that time is an important factor in ameliorative MgO NPs and ZnO NPs effects on elements disruption induced by stress, but their exact interaction with stress systems containing ions level changes needs to more investigation.