Isolation and Identification of extremophiles producing anticancer L-glutaminase and L-Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran

L Glutaminase and L- methioninase, therapeutic enzyme obtained from microbial source have been considered in the treatment of cancer. A total 20 water samples were obtained from the Yale Gonbad hot spring in Qazvin province, Iran. Production capabilities L Glutaminase and Lmethioninase were tested on m9 and mcd plates, respectively. Enzymeproducing isolates were identified by conventional test and then confirmed using molecular methods. The PCR amplicons were directed for sequencing and Phylogenetic tree were drawn using Mega version 6 software. Twelve colonies were obtained from all tested samples. Three extratermophilic isolates showed L-glutaminase activity in M9 medium but only 1 isolates have L-methioninase activity in MCD medium. The phylogenetic tree drawn from the 16 S rRNA gene sequence showed were 100% similar to B. licheniformis and strains of S. epidermidis and L. sphaericus. In this study, Bacillus licheniformis was identified as a dominant and a native strain for the production of L-glutaminase and Lmethioninase.