Author/Authors :
Bahrami, Naghmeh Tehran University of Medical Sciences, Tehran , Bayat, Mohammad Tehran University of Medical Sciences, Tehran , Ai, Armin Dentistry Faculty - Tehran University of Medical Sciences, Tehran , Khanmohammadi, Mehdi Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Ai, Jafar Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Ahmadi, Akbar School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Salehi, Majid Department of Tissue Engineering - School of Medicine - Shahroud University of Medical Sciences, Shahroud , Ebrahimi-Barough, Somayeh Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Goodarzi, Arash Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Karimi, Roya Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Mohamadnia, Abdolreza Department of Biotechnology - School of Advanced Technologies in Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Rahimi, Azam Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
The efficacy of Alginate/ Polyvinyl alcohol/ Hydroxyapatite nanopowders (Alg/PVA/HANPs) mats was investigated for increasing periodontal
ligament stem cells (PDLSCs) differentiation into the osteoblast cells. The Alg/PVA/HANPs nanofibers were fabricated
through electospinning process and following ionic crosslinking of Ca+2 and glutaraldehyde crosslinkers. The physical features
of mats and expression of osteoblast cell markers were evaluated. Results showed the diameter of nanofiber increases with decreasing
the incorporation of HANPs in Alg/PVA nanofiber. According to MTT assay the viability of cells on scaffolds are better in the all of
groups, so the scaffolds were not toxic for the cultured cells. The osteogenic culture media appropriately enhanced osteopontin and
osteocalcin markers. Furthermore, in vitro study indicated that the Alg/PVA/HANPs nanofiber provided a suitable three-dimensional
structure for osteoblast cells differentiation. Thus, the Alg/PVA/HANPs scaffold can be recommended for in bone repair.
Keywords :
Periodontal Ligament Stem Cells , Alginate/ Polyvinyl Alcohol/ Hydroxyapatite , Osteoblast Cells , Nanofiber Scaffold
