Author/Authors :
Sylviana, Nova Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Helja, Niswan Central Laboratories - Faculty Medicine Universitas Padjadjaran, Bandung, Indonesia , Qolbi, Hadaral Hudanul Central Laboratories - Faculty Medicine Universitas Padjadjaran, Bandung, Indonesia , Goenawan, Hanna Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Lesmana, Ronny Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Syamsunarno, Mas Rizky A A Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Agustina, Hasrayati Department of Pathology Anatomy - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Tiksnadi, Badai Batara Department of Cardiology and Vascular Medicine - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Megantara, Imam Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Tarawan, Vita Murniati Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Setiawan, Setiawan Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Hernowo, Bethy Department of Pathology Anatomy - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia , Supratman, Unang Central Laboratories - Faculty Medicine Universitas Padjadjaran, Bandung, Indonesia , Purba, Ambrosius Department of Biomedical Sciences - Faculty of Medicine - Universitas Padjadjaran, Bandung, Indonesia
Abstract :
Background: Hypoxia caused by exercise condition may alter PGC-1α and HIF-1α expression in cardiac cells. Both factors are transcriptional
factors which play a role in conserving energy during metabolic process. However, there is less information about the
effects of long term moderate intensity exercise such as swimming on PGC-1α and HIF-1α expression in cardiac cells.
Objectives: Aim of this study is to observe the effect of force swimming exercise on the expression PGC-1α and HIF-1α gene expression
in cardiac muscle of mice.
Methods: Male BALBc mice were divided into two groups (n = 10): control (C) and swimming (ST). ST group was subjected to moderate
intensity swimming for 4 weeks, 30 minutes/day for 5 days. Hearts of animal models were preserved for cardiac morphology
study using HE staining, PGC-1α and HIF-1α mRNA expression study.
Results: We observed that cardiac PGC-1α expression in ST was significantly increased 1.5 fold compared with control group (0.64
vs 0.44; CI = 95%, -0.378 - 0.019; P = 0.035), but expression of HIF-1α was significantly decreased 0.4 times lower than control group
(0.35 vs 0.52; CI = 95%, 0.27 - 0.31; P = 0,025).
Conclusions: Taken together, ST group increased the expression of PGC-1α but decreased the expression of HIF-1α in mice cardiac
muscle in response to chronic hypoxia condition.
