Author/Authors :
BOZORGOMID, Arezoo Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , NAZARI, Naser Dept. of Medical Parasitology and Mycology - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah , RAHIMI, Hamzeh Dept. of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran, Tehran , BEIGOM KIA, Eshrat Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , HAJJARAN, Homa Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , MOHEBALI, Mehdi Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , ARYAEIPOUR, Mojgan Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , HEIDARIAN, Peyman Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , EZATI, Mohammad Saeid Dept. of Food Hygiene - Faculty of Veterinary Medicine - Science and Research Branch - Islamic Azad University, Tehran , ROKNI, Mohamad Bagher Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background: We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.
Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.
Results: In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sam-ple. No hybrid forms were detected in the present study.
Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran.
Keywords :
Fasciola hepatica , Fasciola gigantica , Genotype , PCR , Iran
