Title of article :
The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods
Author/Authors :
Farzamfar, Bardia Department of Medical Biochemistry - School of Medicine - Tehran University of Medical Sciences, Tehran , Bayanolhagh, Saeed Pasteur Institute of Iran - Biotechnology Process Development Center, Tehran , Mahboudi, Fereidoun Pasteur Institute of Iran - Biotechnology Process Development Center, Tehran , Zahrai, Mahin Department of Medical Biochemistry - School of Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared conjugated molecule, Horseradish peroxidase-Bovine serum albumin- Aflatoxin B1 (HRP-BSA-AFB1). Stabilizing effects of six different stabilizers were compared during 10 months verification at room temperature and 6 weeks verification at 37°C. Based on the results, it was concluded that trehalose-containing stabilizers especially those contain casein in their compositions show the best stabilizing effects on HRP-BSA-AFB1 conjugated tracer.
Keywords :
Aflatoxin , BSA , Conjugate , HRP , Immunoassay , Stabilizer
Journal title :
Astroparticle Physics
