Author/Authors :
Bazzaz, Navid Dept. of Pathology - Shariati Hospital - Tehran University of Medical Sciences - Tehran, Iran , Zare -Mirzaie, Ali Tehran University of Medical Sciences - Tehran, Iran , Nouraee, Nazila Dept. of Experimental Therapeutics - MD Anderson Cancer Center - University of Texas, Houston, Texas , Shahali, Maryam Dept. of Quality Control - Pasteur Institute of Iran - Tehran, Iran , Mowla, Jawad Dept. of Quality Control - Pasteur Institute of Iran - Tehran, Iran , Vasei, Mohammad Cell Based Therapies Research Center - Digestive Disease Research Institute - Shariati Hospital - Tehran University of Medical Sciences - Tehran, Iran
Abstract :
Wilms’ tumor (WT) is the most common genitourinary
tract tumor in children. MicroRNAs (miRNAs) are small non-coding RNAs; their
role in the pathogenesis of many types of human cancers has been identified. We
aimed to evaluate the expression of miR-21, a well-known oncomir, in WT tissue
samples which is a very common urinary tract malignancy in children.
Methods: We performed chromogenic in situ hybridization (CISH) to detect the
sub-cellular localization of miR-21 in 25 formalin-fixed, paraffin-embedded (FFPE)
samples of WT. We also evaluated miR-21 expression in 24 of these blocks and 6
normal kidneys as controls using quantitative real-time PCR technique.
Results: While our real-time PCR analysis showed miR-21 significant
overexpression in 4 tumors compared to the normal kidney samples, we could not
detect significant ISH signal in any of these samples.
Conclusion: Low expression of miR-21 in WT might pinpoint the weak
involvement of this miRNA in the pathogenesis of this cancer.
