Investigation of Antioxidant Properties of Polygonatum orientale Desf and Tilia dasystyla Extracts by Different Methods and Solvents

Background: Free radicals cause many diseases in humans. Antioxidants reduce the risk of cardiovascular disease and stroke by neutralizing free radicals and on the other hand, prevent the progression of cancer. The natural antioxidant enhances the antioxi-dant properties of plasma to prevent diseases such as heart disease, cancer, and stroke. Secondary metabolites derived from plants have a strong potential for antioxidant activity. Methods: The purpose of this study was to investigate the antioxidant activities of two native plants species, including Polygona-tum orientale Desf and Tilia dasystyla extracts, which are obtained with three di erent solvents (water, ethanol, and methanol) and two extraction methods (sonication and water bath) by DPPH (2,2-Diphenyl-1-Picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Potential) methods. This study is the first research to investigate the antioxidant properties of Polygonatum orientale Desf and Tilia dasystyla. Results: The results showed that methanol solvent had a higher eÿciency among the other solvents in extracting of antioxidant compounds. Also, antioxidant level of Tilia dasystyla was higher than Polygonatum oriental Desf. In this regard, the highest FRAP values of Tilia dasystyla belonged to methanol solvent and water bath method with the amount of 2.72 ± 0.36 (mM). Whereas the highest FRAP values of Polygonatum orientae Desf belonged to methanol solvent and sonication method with the amount of 0.147± 0.01 (mM). Conclusions: Both plants showed antioxidant properties but Tilia dasystyla had higher antioxidant activities than Polygonatum ori-entale Desf. Methanol was more e ective solvent in extracting antioxidant compounds and also water bath method was generally an e ective method for extraction.