Title of article :
Development and Validation of a Rapid Derivative Spectrophotometric Method for Determination of Tropicamide in Eye Drops
Author/Authors :
Souri, Effat Department of Medicinal Chemistry - Faculty of Pharmacy and Pharmaceutical Research Center - Tehran University of Medical Sciences , Amanlou, Massoud Department of Medicinal Chemistry - Faculty of Pharmacy and Pharmaceutical Research Center - Tehran University of Medical Sciences , Shahbazi, Shiva Department of Medicinal Chemistry - Faculty of Pharmacy and Pharmaceutical Research Center - Tehran University of Medical Sciences , Bayat, Mitra Department of Medicinal Chemistry - Faculty of Pharmacy and Pharmaceutical Research Center - Tehran University of Medical Sciences
Abstract :
Tropicamide is an antimuscarinic agent used as eye drops for refractive
examinations. The aim of this study was to develop a simple and suitable analytical
method for determination of tropicamide in eye drops in the presence of excipients.
A zero-crossing third and fourth derivative spectrophotometric method was described
for determination of tropicamide in eye drops. The measurements were carried out
at wavelengths of 263.8 and 255.4 nm for third- and fourth-derivative, respectively.
The method was found to be linear (r2> 0.999) in the range of 10-100 g/ml for
tropicamide in the presence of excipients. The limit of determination was 10 mg/ml
for tropicamide. The method was successfully applied for determination of
tropicamide in eye drops without any interference from excipients or need to prior
separation before analysis.
Keywords :
Derivative spectrophotometry , Eye drop , Tropicamide , Validation , Zerocrossing
Journal title :
Astroparticle Physics
