Title of article :
New Usage of a Fluorometric Method to Assay Antioxidant Activity in Plant Extracts
Author/Authors :
Moein, Soheila Department of Biochemistry and Molecular Medicine Research Center - Hormozgan University of Medical Sciences, Bandar Abbas , Moein, Mahmood Reza bedicinal Plant Research Center - and Department of Pharmacognosy - School of Pharmacy, Shiraz University of Medical Sciences
Abstract :
In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein
(LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of
antioxidant properties of plant extracts. The rate of the reaction was followed by
monitoring the formation of DCF as a function of time. The antioxidant assay was
carried out for different concentrations of gallic acid, Salvia mirzayanii, Rech. f &
Esfand Phlomis persica Boiss crude extracts and their fractions obtained by thin layer
chromatography (TLC). The results showed that the fluorometric method could be
used to detect lower concentrations of antioxidants. Thus, we were able to identify
the antioxidant activity of five fractions obtained by TLC of Salvia mirzayanii extract
and four fractions in TLC of Phlomis persica extract. This method is a good
candidate to be used in high throughput screening.
Keywords :
Antioxidant activity , Dichlorofluorescein , Fluorometric method , Phlomis persica , Salvia mirzayanii , Thin layer chromatography
Journal title :
Astroparticle Physics
