Author/Authors :
Khanra, Kalyani Department of Biotechnology - Panskura Banamali College, Panskura RS, Purba Medinipur, West Bengal, India , Choudhuri, Indranil Department of Biotechnology - Panskura Banamali College, Panskura RS, Purba Medinipur, West Bengal, India , Bhattacharyya, Nandan Department of Biotechnology - Panskura Banamali College, Panskura RS, Purba Medinipur, West Bengal, India
Abstract :
Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene
consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in
deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was
isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect
mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a
point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis
showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. Conclusion: This is
a newly reported somatic mutation of pol β in ovarian carcinoma patients from India.
