Title of article :
DNA Binding Study of Dihydropyrano [3, 4-C] Chromene Derivative by some Spectroscopic Techniques
Author/Authors :
Farajzadeh Dehkordi, Mahvash Department of Biology - Faculty of Natural Sciences, University of Tabriz , Dehghan, Gholamreza Department of Biology - Faculty of Natural Sciences, University of Tabriz , Mahdavi, Majid Department of Biology - Faculty of Natural Sciences, University of Tabriz , Hosseinpour Feizi, Mohammad Ali Department of Biology - Faculty of Natural Sciences, University of Tabriz
Abstract :
This study was designed to examine the interaction of
dihydropyranochromene derivative, 2-amino-4-phenyl-5-oxo-4H, 5Hpyrano-[
3, 2-c] chromene-3-carbonitrile (4-PC) with calf thymus DNA
(ctDNA) by absorption spectroscopy, competitive fluorescence studies,
circular dichroism (CD) and viscosity measurements. It was found that 4-PC
could interact with ctDNA through non- intercalative mode and the intrinsic
binding constant, Kb, for 4-PC with ctDNA was estimated to be 2.74 ×103 M-
1. Methylene blue (MB) displacement studies revealed that 4-PC could not
replace ctDNA bounded MB, which is indicative of groove binding mode of
4-PC with DNA. Furthermore, 4-PC induced detectable changes in the CD
spectrum of ctDNA as well as changes in its viscosity, corroborate the above
experimental results.
Keywords :
Binding constant , Chromone derivative , Circular dichroism , Groove binding
Journal title :
Astroparticle Physics
