Title of article :
Effect of Tribulus Terrestris Aqueous Extract on Survival and Growth of Human Peripheral Blood Mononuclear Cells (Hpbmc) and Several Cancerous Cell Lines
Author/Authors :
Mohamed khosroshahi, Lida Department of immunology - School of Medicine - Kermanshah University of Medical Sciences , Nikoo, Marziyeh Department of immunology - School of Medicine - Kermanshah University of Medical Sciences , Hasanpoor, Zahra Department of immunology - School of Medicine - Kermanshah University of Medical Sciences , Mostafaie, Ali Medical Biology Research Center - Kermanshah University of Medical Sciences
Abstract :
Tribulus terrestris (TT) is a member of the Zygophyllaceae family.
Traditional Chinese medicine recommends the use of TT for treatment of
a variety of diseases. In the present study we further investigated antiproliferative
and cytotoxic effects of TT extract on normal and several
malignant cell lines. For this purpose, TT was collected and after
authentication, aqueous extract was prepared. Cytotoxic activity of TT
extract at different doses (5-640μg/ml) was determined by LDH
assay. Proliferative activity of TT extract was determined by trypan blue
staining and cell counting methods. The results showed that IC50 of the
extract for cancer cell line is 320 μg/ml and it is higher for hPBMC. On the
other hand, we observed proliferative effect of TT extract on hPBMC but
not on malignant cell lines.
Keywords :
Cell line , Growth , Survival , Tribulusterrestris
Journal title :
Astroparticle Physics
