Title of article :
Free Radical Scavenging Activity and Total Phenolic Content of Methanolic Extracts from Male Inflorescence of Salix aegyptiaca Grown in Iran
Author/Authors :
Sonboli, Ali Department of Biology - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran , Mojarrad, Mehran Department of Biology - Payame Noor University, Naqadeh , Nejad Ebrahimi, Samad Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran , Enayat, Shabnam Department of Biology - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran
Abstract :
This study was designed to examine the in vitro antioxidant activities and total phenolic contents of the methanolic extracts from male inflorescence of Salix aegyptiaca L. grown in Iran. The methanolic extract (ME) and its three fractions including water (WF), butanol (BF) and chloroform (CF) were prepared and then their antioxidant activities, as well as total phenolic contents, were evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the Folin–Ciocalteu method, respectively. Among the different fractions of methanol extract, BF indicated the most antioxidant activity with an IC50 value of 27.7 μg/mL and total phenols of 313.8 ppm, which is comparable with the synthetic antioxidant BHT (IC50 = 26.5μg/mL). The antioxidant activities of the other fractions decreased in the order of ME >WF > CF. The potent antioxidant activity of S. aegyptiaca supported its possible use as a natural antioxidant in food industries and other pharmaceutical preparations.
Keywords :
Free radical scavenging activity , DPPH , Total phenolic contents , Methanol extracts , Salix aegyptiaca , Iran
Journal title :
Astroparticle Physics
