Title of article :
Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
Author/Authors :
LU ، Yizhuo Department of General Surgery - Zhongshan Hospital, Institute of Gastrointestinal Oncology, School of Medicine - Xiamen University , LI ، Lianghui Department of General Surgery - Zhongshan Hospital - Xiamen University , WU ، Guoyang Department of General Surgery - Zhongshan Hospital - Xiamen University , ZHUO ، Huiqin Institute of Gastrointestinal Oncology, School of Medicine - Xiamen University , LIU ، Guoyan Department of Gastrointestinal Surgery - Zhongshan Hospital - Xiamen University , CAI ، Jianchun Department of Gastrointestinal Surgery - Institute of Gastrointestinal Oncology, School of Medicine, Zhongshan Hospital - Xiamen University
Abstract :
Background: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells. Methods: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly. Results: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway. Conclusion: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors.
Keywords :
Gastric cancer , LY294002 , MK , 2206 , Phosphorylation , PI3K , Akt signaling pathway , PRAS40 , Thr246
Journal title :
Iranian Journal of Public Health
Journal title :
Iranian Journal of Public Health
