Title of article :
A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain
Author/Authors :
Sekhavati, Mohammad Department of Bacteriology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran , Mohabati Mobarez, Ashraf Department of Bacteriology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran , Siadat, Davar Microbiology Research Center (MRC) Pasteur - Institute of Iran, Tehran , Noofeli, Mojtaba Department of Human Bacterial Vaccine - Razi Vaccine and Serum Research Institute - Agricultural Research - Education and Extension Organization (AREEO), Karaj
Abstract :
Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of
acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding
researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications,
making them a suitable vaccine candidate against pertussis.
Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron
microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs.
Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous
hemagglutinin, and pertactin was verified using Western blot and ELISA assay.
Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be
utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.
Keywords :
Bordetella pertussis , Extraction , Outer membrane vesicle , Vaccine
Journal title :
Astroparticle Physics
