Author/Authors :
Hossein Aghdaie, M Shiraz University of Medical Sciences, Shiraz , Azarpira, N Shiraz University of Medical Sciences, Shiraz , Esfandiari, E Shiraz University of Medical Sciences, Shiraz , Kaviani, M. Shiraz University of Medical Sciences, Shiraz , Golbabapour, S Shiraz University of Medical Sciences, Shiraz , Shamsaeefar, A Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Kazemi, K Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Dehghani, M Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Bahador, A Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Salahi, H Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Nikeghbalian, S Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Malek-Hosseini, S. A Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Geramizadeh, B Department of Pathology - Shiraz University of Medical Sciences, Shiraz
Abstract :
Background: Liver transplantation is the only treatment for end-stage and genetic liver diseases. The
main burden of this treatment is the shortage of both living and cadaveric liver donors. An alternative
treatment is using liver cell transplantation, which can be obtained from unused livers for transplantation.
These hepatocytes should be kept ready in viable and functional situation in a frozen state to be
instantly used when they would be needed. In our previous experience, we had isolated hepatocytes from
unused livers.
Objective: To find a preserving solution for increasing viability and function of the isolated hepatocytes
that are stored to be transplanted.
Methods: 9 cadaveric donor livers, which were not used for transplantation due to various causes such
as severe steatosis, were selected to isolate hepatocytes. Various cold storage solutions were tried to find
the best temperature for more viability and functionality for preservation of hepatocytes. University of
Wisconsin (UW) solution and Williams E media were used as control media. 2 anti-apoptotic and antioxidative
solutions, i.e., α-lipoic acid and ursodeoxycholic acid (UDCA), were used as cold preservatives
solutions. The numbers of viable hepatocytes were estimated by trypan blue method; the functionality
was assessed by the cells ability to produce urea.
Results: The highest number of viable and functional hepatocytes was obtained from freshly isolated cells.
However, after preservation, the number of these viable hepatocytes and their functionality were not
significantly different in cold storage solutions comparing to the control media used. Functionality of
the isolated hepatocytes stored in UW with and without UCDA solution was similar to freshly isolated
hepatocytes.
Conclusion: Preservatives with anti-apoptotic and antioxidant activity could not increase the number of
viable hepatocytes. Functionality of cold storing hepatocytes could be preserved similar to freshly isolated
hepatocytes by UW solution with and without UCDA.
Keywords :
Transplantation , Viability , Cold storage , Preservation
