Title of article :
Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015-16
Author/Authors :
IZADI, Shahrokh Department of Medical Parasitology and Mycology - School of Medicine - Isfahan University of Medical Sciences , MOHAGHEGH, Mohammad Ali Department of Laboratory Sciences - School of Paramedical Sciences - Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh , GHAYOUR-NAJAFABADI, Zahra Department of Medical Parasitology and Mycology - School of Medicine - Isfahan University of Medical Sciences , AZAMI, Mehdi Skin Diseases and Leishmaniasis Research Center - Isfahan University of Medical Sciences , MIRZAEI, Farzaneh Department of Medical Parasitology and Mycology - School of Medicine - Shahid Sadoughi University of Medical Sciences , NAMDAR, Fatemeh Department of Medical Parasitology and Mycology - School of Medicine - Isfahan University of Medical Sciences , MOHEBALI, Mehdi Department of Medical Parasitology - School of Public Health - Tehran University of Medical Sciences , LESHAN WANNIGAMA, Dhammika Department of Microbiology - Faculty of Medicine - Chulalongkorn University - King Chulalongkorn Memorial Hospital, Thailand , HEJAZI, Hossein Department of Medical Parasitology and Mycology - School of Medicine - Isfahan University of Medical Sciences
Abstract :
Background: The purpose of this study was to determine the prevalence and genotype of
Cryptosporidium spp. in different groups of immunocompromised patients admitted to the
referral hospitals in center of Iran during 2015–2016.
Methods: This cross-sectional study was conducted on 346 immunocompromised patients
(HIV+/AIDS, Lymphoma, Leukemia and organ transplants) in referred hospitals from central
parts of Iran including Isfahan, Markazi, Yazd and Chaharmahale Bakhtiari provinces.
Stool samples were analyzed for Cryptosporidium species, modified Ziehl–Neelsen staining
techniques followed by the semi-nested PCR and DNA sequencing methods.
Results: The total rate of Cryptosporidium spp. was 3.46% (12/346) in the patients, however,
the prevalence of the parasite, was 4.6% (4/87) in HIV+/AIDS patients, 3.6% (6/168) in
patients with blood malignancy and 2.1% (2/91) in organ transplant recipients. The SSU
rRNA gene of Cryptosporidium spp. in all microscopic-positive samples was effectively amplified
by the semi-nested PCR and DNA sequences, exposed the existence of two Cryptosporidium
species, including C. hominis 91.6% (11/12) and C. parvum 8.3% (1/12).
Conclusion: The predominance of C. hominis in the present study may be certifies the importance
of anthroponotic transmission of cryptosporidiosis in center of Iran.
Keywords :
Cryptosporidium , Immunocompromised patients , Genotype , Iran
Journal title :
Astroparticle Physics
