Author/Authors :
Fatemikia, Hossein Department of Physiology - School of Medicine - Shiraz University of Medical Sciences, Shiraz , Kamyab, Mostafa Department of Aquatic Biotechnology - Faculty of Life Sciences and Biotechnology - Shahid Beheshti University, Tehran , Movahed, Ali The Persian Gulf Tropical Research Center - Biochemistry Group - Bushehr University of Medical Sciences, Bushehr , Sadeghi, Mehdi Department of Physiology - School of Medicine - Bushehr University of Medical Sciences, Bushehr , Kim, Euikyung College of Veterinary Medicine - Gyeongsang National University - Jinju, South Korea , Behdani, Mahdi Biotechnology Research Center - Venom and Biotherapeutics Molecules Laboratory - Pasteur Institute of Iran, Tehran , Mohammadpour Dounighi, Naser Department of Human Vaccine and Serum - Razi Vaccine and Serum Research Institute - Agricultural Research - Education and Extension Organization, Karaj , Shahrivar, Mehrnaz School of Medicine - Bushehr University of Medical Sciences, Bushehr , Seyedian, Ramin Department of Pharmacology - Bushehr University of Medical Sciences, Bushehr
Abstract :
Objective(s): In this study, the neutralizing abilities of the equine and the recently introduced camelid
antivenoms on the hemodynamic parameters (inotropism, chronotropism, and arrhythmogenicity)
were assessed following envenomation by Hemiscorpius lepturus venom in rats.
Materials and Methods: At first, the electrophoretic profiles of both products were obtained by using the
SDS-PAGE method (12.5%) and stained with Coomassie blue and silver nitrate. Secondly, different doses of
the camelid antivenom (10, 50, and 100 μl) were given intravenously in 10 min before venom injection (400
μg/rat). The neutralizing potencies of camelid and equine antivenoms were measured by preincubation
(100 μl) with H. lepturus venom for 30 min at room temperature. Finally, equal amounts of the antivenoms
were injected intravenously to observe the hemodynamic changes.
Results: Based on the electrophoretic profile, it was evident that undesired proteins significantly
decreased in equine antivenom, owing to impurities. Pretreatment with the camelid antivenom (100
μl), neutralized the elevation of the mean arterial pressure evoked with scorpion venom injection
(88.15±4.56 versus 10.2±1.23 percent at the 8th min). The Incubation of the venom and the camelid
antivenom counteracted the hemodynamic changes, but the equine product had no effect. The
intravascular injection of the equine antivenom transiently increased the mean arterial pressure as
compared to the control (108.67±8.63 mmHg versus 52.67±1.93 mmHg at the 10th min).
Conclusion: The most obvious finding emerging from this study was that the camelid antivenom
neutralized the hemodynamic changes in rats significantly, but in comparison, the equine antivenom
had just a minor ability.
Keywords :
Camelid antivenom , Cardiovascular , Envenomation , Equine antivenom , Hemiscorpius lepturus
