Title of article :
Purification of Sesquiterpenes from Saussurea Lappa Roots by High Speed Counter Current Chromatography
Author/Authors :
Cao, Kun College of Chemistry and Chemical Engineering - Chongqing University, Chongqing P.R. China , Qian, Wei College of Chemistry and Chemical Engineering - Chongqing University, Chongqing P.R. China , Xu, Yi College of Chemistry and Chemical Engineering - Chongqing University, Chongqing P.R. China , Zhou, Zhen College of Chemistry and Chemical Engineering - Chongqing University, Chongqing P.R. China
Abstract :
Sesquiterpenes lactones including costunolide and dehydrocostus lactone, were isolated
from Saussurea lappa roots, which had exhibited a wide range of biological activities such
as anti-cancer, anti-inflammatory, antiulcer, and immunomodulatory activities. High-speed
counter-current chromatography (HSCCC) was applied for the rapid preparative isolation
of sesquiterpenes lactones from Saussurea lappa roots. A solvent optimization method for
HSCCC was presented, i.e. the separation factors of compounds after the partition coefficient
(K) of solvent system should be investigated. Using this method, 150 mg of costunolide, 140
mg of dehydrocostus and 15 mg of 10-methoxy-artemisinic acid with purities of 95%, 98%,
and 98% were obtained within 150 min. The structures of three compounds were identified
by mass spectrum (MS) and nuclear magnetic resonance (NMR) spectroscopy. These results
offered an efficient strategy for separation of potentially health-relevant phytochemicals from
Saussurea lappa roots.
Keywords :
Orthogonal test , HSCCC , select solvent system , Sesquiterpenes lactones , Saussurea lappa Roots
Journal title :
Astroparticle Physics
