Author/Authors :
Deng, Shiren College of Pharmacy - Liaoning University of Traditional Chinese Medicine, Dalian, China , Xia, Linbo College of Pharmacy - Liaoning University of Traditional Chinese Medicine, Dalian, China , Zhu, Xiamin College of Pharmacy - Liaoning University of Traditional Chinese Medicine, Dalian, China , Zhu, Jiang College of Pharmacy - Liaoning University of Traditional Chinese Medicine, Dalian, China , Ca, Mingchen College of Pharmacy - Liaoning University of Traditional Chinese Medicine, Dalian, China , Wang, Xin College of Pharmacy - Liaoning University of Traditional Chinese Medicine, Dalian, China
Abstract :
An efficient and rapid affinity-based screening method for directly fishing out natural
alpha-glucosidase inhibitors from Cyperus rotundus extract by using immobilized enzyme
technology combined with UHPLC-QTOF MS analysis was established. As a result without
time-consuming and laborious isolation workload and false positive interference, five natural
alpha-glucosidase inhibitors were successfully recognized and identified from only 400 uL
of C. rotundus extracts within only a couple of hours, which suggested that the screening
method was rapid, economical, sensitive and feasible. In addition, the captured compounds
were isolated and characterized as stilbenoids oligomers, and were proved to be strong alphaglucosidase
inhibitors by inhibitory assay in-vitro. Among them, 3 stilbenoids trimers were
reported to be potent α-glucosidase inhibitors for the first time. This method could be modified
and have the potential for rapidly screening of active compounds extracts against some new
targets by immobilizing some other biomacromolecules.
Keywords :
UHPLC-QTOF MS , Stilbenoid timers , Cyperus rotundus , Immobilized enzyme , Alpha-glucosidase inhibitors , Affinity fishing
