Author/Authors :
Khademi, Emad Molecular and Cell Biology Research Center - Department of Anatomy - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari , Pirhajati Mahabadi, Vahid Cellular and Molecular Research Center - Iran University of Medical Sciences, Tehran - Neuroscience Research Center - Iran University of Medical Sciences, Tehran , Ahmadvand, Hassan Department of Biochemistry - Faculty of Medicine - Lorestan University of Medical Sciences, Khorramabad - Razi Herbal Researches Center - Lorestan University of Medical Sciences, Khorramabad , Akbari, Esmaeil Immunogenetic Research Center and Department of Physiology and Pharmacology - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari , Khalatbary, Ali Reza Molecular and Cell Biology Research Center - Department of Anatomy - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari
Abstract :
Objective(s): Cisplatin-induced peripheral neuropathy is a debilitating side effect in patients receiving
this drug. Recent studies suggest hyperbaric oxygen (HBO) therapy as a new treatment approach for
models of neural injury. The aim of the current study was to determine the protective effects of HBO
preconditioning against peripheral neuropathy induced by Cisplatin (CDDP).
Materials and Methods: The present study was conducted on 4 groups of rats: Sham group; HBO
group (60 min/d); Control group (CDDP 2 mg/kg/d); Precondition group (HBO+CDDP). Mechanical
threshold testing was weekly carried out using von Frey filament. Sciatic nerve and associated
ganglia were removed five weeks after the first CDDP injection for biochemical evaluation of
malondialdehyde (MDA) content and myeloperoxidase (MPO) activity, immunohistochemistry of
terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), TNF-α, caspase-3 and iNOS,
and transmission electron microscopic (TEM) assessments.
Results: MDA levels and MPO activities were significantly decreased in preconditioned rats. Attenuated
TUNEL reaction along with attenuated caspase-3, TNF-α, and iNOS expression could be significantly
detected in preconditioned rats. Also, HBO preconditioning improved the nociceptive threshold.
Conclusion: The results suggest that HBO preconditioning can attenuate peripheral neuropathy
caused by cisplatin in rats.
Keywords :
Apoptosis , Cisplatin , Hyperbaric oxygen , Inflammation , Neuropathy
