Title of article :
Efficacy of insulin targeted gene therapy for type 1 diabetes mellitus: A systematic review and meta-analysis of rodent studies
Author/Authors :
Rahimi Ghiasi, Moosa Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences , Mohammadi, Hamed Food Security Research Center - School of Nutrition and Food Science - Isfahan University of Medical Sciences , Symonds, Michael E. The Early Life Research Unit - Division of Child Health - Obstetrics and Gynaecology and Nottingham Digestive Disease Centre and Biomedical Research Centre - School of Medicine - University of Nottingham - Nottingham, UK , Tabei, Mohammad Bagher Department of Genetics & Maternal-Fetal Research Center - Shiraz University of Medical Sciences , Salehi, Ahmad Reza Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences , Jafarpour, Sima Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences , Norouzi Barough, Leila Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences , Rahimi, Elnaz Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences , Amirkhani, Zohreh Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences , Miraghajani, Maryam Cancer Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Salehi, Rasoul Pediatric Inherited Diseases Research Center - Isfahan University of Medical Sciences - Department of Genetics and Molecular Biology - School of Medicine - Isfahan University of Medical Sciences
Abstract :
Diabetes mellitus (DM) is a major worldwide public health challenge, for which gene therapy offers
a potential therapeutic approach. To date, no systematic review or meta-analysis has been published
in this area, so we examined all relevant published studies on rodents to elucidate the overall
effects of gene therapy on bodyweight, intraperitoneal glucose tolerance test (IPGTT), fasting blood
glucose, and insulin in animals with type 1 DM. The Cochrane Library, PubMed, Embase, ISI Web of
Science, SCOPUS, and Google Scholar were systematically searched for potentially relevant studies.
Mean±standard deviation (SD) was pooled using a random-effects model. After the primary search,
out of 528 studies identified, 16 studies were in concordance with predefined criteria and selected for
the final assessment. Of these, 12 studies used viral manipulation, and 4 employed non-viral vectors
for gene delivery. The meta-analysis showed gene therapy with a viral vector decreased mean IPGTT
(-12.69 mmol/l, P<0.001), fasting blood glucose (-13.51 mmol/l, P<0.001), insulin (398.28 pmol/l,
P<0.001), and bodyweight (24.22 g, P<0.001), whereas non-viral vectors reduced fasting glucose
(-29.95 mmol/l, P<0.001) and elevated insulin (114.92 pmol/l, P<0.001). Gene therapy has favorable
effects on alleviating type 1 DM related factors in diabetic rodents.
Keywords :
Gene therapy , Insulin , Meta-analysis , Non-viral vector , Type 1 diabetes mellitus , Viral vector
Journal title :
Astroparticle Physics
