Author/Authors :
Peng, Xiao Department of Immunology - Medical School of Ningbo University - Ningbo 315211, China , Pan, Xiuhe Department of Immunology - Medical School of Ningbo University - Ningbo 315211, China , Tan, Jun Department of Hepatology - HwaMei Hospital - University of Chinese Academy of Sciences - Ningbo 315010, China , Li, Yan Department of Immunology - Medical School of Ningbo University - Ningbo 315211, China , Li, Mingcai Department of Immunology - Medical School of Ningbo University - Ningbo 315211, China
Abstract :
Objective(s): Interleukin-36 receptor antagonist (IL-36Ra) is a new member of the IL-1 family that
exhibits anti-inflammatory activity in a variety of inflammatory and immune diseases. Our purpose
was to determine the effect of IL-36Ra on liver injury in a mouse hepatitis model induced by
concanavalin A (ConA).
Materials and Methods: Mice were treated with IL-36Ra DNA or pcDNA3.1 control plasmid using a
hydrodynamic gene delivery approach.
Results: Our data reveal that treatment with IL-36Ra decreased liver inflammation and serum level
of aminotransferases. Furthermore, IL-36Ra reduced ConA-induced pro-inflammatory cytokines
(interferon-γ, tumor necrosis factor-α, and IL-17A) production when compared to control plasmid.
Conclusion: Our results demonstrated that IL-36Ra is a critical protector against ConA-induced liver
injury.
Keywords :
Concanavalin A (ConA) , Hydrodynamic-based gene - delivery , IL-36Ra , Inflammation , Liver injury , Plasmid
