Title of article :
Evaluation of Th1/Th2 Lymphocyte Balance in Peripheral Blood Mononuclear Cells of Patients with Hashimoto’s Thyroiditis
Author/Authors :
VASEGHI, Hajar Department of Photo Healing and Regeneration - Medical Laser Research Center - Yara Institute - ACECR, Tehran , ESFAHANIAN, Fatemeh Department of Endocrinology - Imam Khomeini Hospital Complex - Tehran University of Medical Sciences, Tehran , JADALI, Zohreh Department of Immunology - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background: The role of T cells in the pathogenesis of Hashimoto’s thyroiditis is well established, whereas the precise and likely the overlapping contributions of different T-cell subpopulations to thyroid injury are less understood. The purpose of this study was to assess the expression pattern of two lineage determining tran-scription factors, T-bet and GATA-3 that regulate differentiation of T cells into Th1 or Th2 cell fates, respec-tively. Moreover, the mRNA expression and plasma concentration of Th1(IFN-γ) and Th2(IL-4) cytokines were analyzed.
Methods: In this case-control study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine the expression patterns of various transcripts in 20 patients (in Endocrinology Clinic, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran, in 2015) with Hashimoto’s thyroiditis (HT) and 22 healthy controls. Plasma IL-4 and IFN-γ concentrations were also meas-ured using enzyme-linked immunosorbent assay.
Results: T-bet gene expression was significantly lower in patients compared to healthy controls (P=0.014). The expression of IL-4 mRNAs was significantly increased in the peripheral blood mononuclear cells from patients as compared to normal controls (P=0.001). In addition, a marked increase in plasma IL-4 levels were observed in patient group compared to controls (P=0.043). Conclusion: Altered balance between Th1 and Th2 related transcription factors and cytokines may be impli-cated in the pathogenesis of Hashimoto’s thyroiditis.
Keywords :
Hashimoto's thyroiditis , T lymphocyte , Autoimmunity , Cytokine
Journal title :
Astroparticle Physics