Author/Authors :
yeganehzad, samira Department of Food Processing - Research Institute of Food Science and Technology, Mashhad, Iran , pahlevanloo, abolfazl Department of Food Processing - Research Institute of Food Science and Technology, Mashhad, Iran , kiumarsi, maryam Department of Food Processing - Research Institute of Food Science and Technology, Mashhad, Iran , shahidi, mostafa Department of Food Processing - Research Institute of Food Science and Technology, Mashhad, Iran , zayerzadeh, azadeh Department of Food Processing - Research Institute of Food Science and Technology, Mashhad, Iran , nadali, narjes Department of Food Processing - Research Institute of Food Science and Technology, Mashhad, Iran
Abstract :
Objective: Some food-derived bioactive peptides have demonstrated positive effects on stress reduction. This study was an attempt to evaluate the effectiveness of daily consumption of 12 g of sugar-free chocolate containing 150 mg of αs1-casein (91-100) peptide in alleviating stress in healthy, normal-weight participants.
Methods: Salivary cortisol concentration and self-report questionnaire data were obtained before and after chocolate consumption. Sixty participants completed the Cattle Anxiety Scale (CSI), the Beck Depression Inventory (BDI), and the Depression Anxiety Stress Scales 21 (DASS-21).
Results: Results from the CAS and DASS-21 questionnaires showed that consumption of chocolate containing bioactive peptide reduced anxiety and stress (p < 0.05). Salivary cortisol measurement showed more than 30% reduction in cortisol level in the intervention group. The peptide had a 95% recovery rate and also demonstrated thermal and mechanical stability during the production process.
Conclusion: Findings confirmed the undenatured structure of the αs1-casein peptide in chocolate and its resistance to the chocolate processing condition.
Keywords :
Bioactive peptide , Sugar-free chocolate , Stress , salivary cortisol
