Author/Authors :
sefati, niloofar Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , abbas zadeh, hojjat allah Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Fadaei Fathabady, fatemeh Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , abdollahi far, mohammad amin Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , norouzian, mohsen Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Introduction: Many studies have shown the positive effect of laser radiation and application of the mesenchymal stem cells (MSCs) and their secretion in stimulating bone regeneration. The aim of this study was determining effects of MSC conditioned media (CM) and low-level laser (LLL) on healing bone defects in the hypothyroid male rat.
Methods: We assigned 30 male Wistar rats randomly to 3 groups: control, hypothyroidism, CM+LLL. Four weeks after surgery, the right tibia was removed. Biomechanical examination and histological examinations were performed immediately.
Results: Our results showed significant increase in bending stiffness (116.09 ±
18.49), maximum force (65.41 ± 8.16), stress high load (23.30 ± 7.14), energy absorption (34.57 ± 4.10), trabecular bone volume (1.34 ± 0.38) and the number of osteocyte, osteoblast, and osteoclast (12.77 ± 0.54, 6.19 ± 0.80, 1.12 ± 0.16 respectively) in osteotomy site in the LLL + CM group compared to the hypothyroidism group (P < 0.05).
Conclusion: The results indicated that using the LLL + CM may improve fracture regeneration and it may hasten bone healing in the hypothyroid rat.
