Author/Authors :
Osman, Najem Aldin Mohammed Department of Biotechnology - Faculty of Science and Technology - Omdurman Islamic University, Sudan , Elhag Elrayah, Intisar College of Applied Medical Science - Shaqra University , Altayb, Hisham Department of Microbiology - College of Medical Laboratory Sciences - Sudan University for Science and Technology , Elhaj, Nihad Mohammed Department of Microbiology - Tropical Medicine Research Institute - National Center for Research, Sudan , Salih, Mohamed Ahmed Department of Biotechnology - Biotechnology Park Africa City of Technology, Sudan , Abuzeid, Nadir Faculty of Medical Laboratory Sciences - Omdurman Islamic University , Eldirdery, Muataz Mohmed Department of Microbiology - Tropical Medicine Research Institute - National Center for Research, Sudan , Mahgoub Mohamed, Yassir Department of Microbiology - Tropical Medicine Research Institute - National Center for Research, Sudan
Abstract :
Staphylococcus aureus carrying PVL gene remain major health problem associated with highly virulent infections. Characterization of such gene is important to know the impact and the functional significance of nucleotide variations. PCR and standard sequencing were performed for twelve Sudanese strains from different sources. Protein structures prediction, modeling and physiochemical analysis were done to analyze genetic variation in PVL. Single nucleotide polymorphism at position 610 (A / G) of lukS-PV was detected in all tested isolates resulted in nonsynonymous mutation in the amino acid sequence at position 204 (N / D). Using molecular and bioinformatics tools the detected novel mutation in lukS-PV may have a diagnostic functional significance.
Keywords :
S. aureus , PVL , lukS-PV , Mutation , Sudanese
