Author/Authors :
Khosravi, Maryam Bionorganic Chemistry Research Laboratory - Chemistry Department - Iran University of Science and Technology, Narmak, Tehran , Rahimi, Rahmatollah Bionorganic Chemistry Research Laboratory - Chemistry Department - Iran University of Science and Technology, Narmak, Tehran , Rabbani, Mahboubeh Bionorganic Chemistry Research Laboratory - Chemistry Department - Iran University of Science and Technology, Narmak, Tehran , Safavi, Ebrahim World Society of the Anti-Aging Medicine and International Hormone Society, Niavara, Tehran
Abstract :
Peptides have shown Promising effect as pharmaceutics with the potential to treat a wide variety of diseases. Peptides are mostly synthesized by biological technology or chemical methods. Solution phasepeptide synthesis (SPS) and solid phase peptide synthesis (SPPS) are two major chemical techniques for peptidesproduction.In this research, the synthesis of dipeptde(β-alanine-L-histidine)wasexaminedby the both SPS and SPPS methods. The solution phase synthesizeswasdonebyN-phthalic anhydride as a protective group andthe solid phase using standardtert-Butyloxycarbonyl (BOC) and Fluorenylmethyloxycarbonylchloride (Fmoc).Inthe both methods,themicrowave was assisted in different steps. The molecular structure of the dipeptide(β-alanine-L-histidine) wasdefined using different spectrometrymethods such as:UV-Vis, FT-IR, 1HNMR and LC-Massanalysis.
Keywords :
Peptide , Microwave , Solution phase peptide synthesis , Solid phase peptide synthesis , Liquid chromatography–mass spectrometry
