Other language title :
ﺟﻤﻌﯿـﺖﻫـﺎي Varroa destructor (Acari: Varroidea) ﺟﻨـﻮب اﯾـﺮان ﺑـﻪ ﻫﺎﭘﻠﻮﺗﯿـﭗ K از ﺳﯿﺘﻮﮐﺮوم اﮐﺴﯿﺪاز زﯾﺮواﺣﺪ ﯾﮏ ﻣﯿﺘﻮﮐﻨﺪرﯾﺎﯾﯽ ﺗﻌﻠﻖ دارﻧﺪ
Title of article :
Varroa destructor (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI
Author/Authors :
Hajializadeh, Zahra Department of Plant Protection - College of Agriculture - Shahid Bahonar University of Kerman , Asadi, Mahdieh Department of Plant Protection - College of Agriculture - Shahid Bahonar University of Kerman , Ahmadi, Kamal Department of Plant Protection - College of Agriculture - Shahid Bahonar University of Kerman , Balvasi, Asieh Department of Plant Protection - Qaemshahr Branch - Islamic Azad University
Abstract :
A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.
Farsi abstract :
وﯾﮋﮔﯽﻫﺎي ﻣﻮﻟﮑﻮﻟﯽ ﺑﺮ اﺳﺎس ژن ﺳﯿﺘﻮﮐﺮوم اﮐﺴﯿﺪاز زﯾﺮواﺣﺪ ﯾﮏ ﻣﯿﺘﻮﮐﻨﺪرﯾﺎﯾﯽ ﺑﺮاي ژﻧﻮﺗﯿﭗ ﺟﻤﻌﯿﺖﻫﺎي Varroa destructor ﻣﺮﮐﺰ-ﺟﻨﻮب اﯾﺮان ﺑﺮاي ﻧﺨﺴﺘﯿﻦ ﺑﺎر ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺖ. 570 ﺟﻔﺖ ﺑﺎز ﺑﺎ واﮐﻨﺶ زﻧﺠﯿﺮهاي ﭘﻠﯿﻤﺮاز ﺗﮑﺜﯿﺮ و ﺑﺎ دو آﻧﺰﯾﻢ ﺑﺮﺷﮕﺮ ﻫﻀﻢ ﺷﺪ. ﻫﻀﻢ ﻣﺤﺼﻮل ﭘﯽ ﺳﯽ آر ﺑﺎ XhoI ﻗﻄﻌﺎت 300 و 270 ﺟﻔﺖ ﺑﺎز در ﻫﻤﻪ ﻧﻤﻮﻧﻪﻫﺎ ﺗﻮﻟﯿﺪ ﮐﺮد، درﺣﺎﻟﯽﮐﻪ ﻫﻀﻢ ﺑﺎ SacI ﻫﯿﭻ ﻗﻄﻌﺎت ﻫﻀﻢ ﺷﺪهاي ﺗﻮﻟﯿﺪ ﻧﮑﺮد. ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ ﺗﻤﺎم ﻧﻤﻮﻧﻪﻫﺎ ﻣﺘﻌﻠﻖ ﺑﻪ ﻫﺎﭘﻠﻮﺗﯿﭗ K ﺑﻮده و ﭘﯿﺶﺗﺮ ﺑﺮاي V. destructor در ﻣﻨﺎﺑﻊ ذﮐﺮﺷﺪه اﺳﺖ. در ﻣﺠﻤﻮع 22 ﺗﻮاﻟﯽ ﻧﻤﻮﻧﻪﻫﺎي اﯾﺮاﻧﯽ )418bp ﺑﺮاي ﻧﺨﺴﺘﯿﻦ ﺑﺎر در NCBI ﺛﺒﺖ ﺷﺪﻧﺪ.
Keywords :
Genetic diversity , haplotype , Mesostigmata , mitochondrial genome , molecular characterization
