Other language title :
ﮔﻮﻧﻪﻫﺎي ﺟﻨﺲ Hyalomma (Acari: Ixodidae) اﯾﺮان و ﺷﻮاﻫﺪ ﻣﻮﻟﮑﻮﻟﯽ ﺑﺮاي درك وﺿﻌﯿﺖ ﺗﺎﮐﺴﻮﻧﻮﻣﯿﮏ ﮐﻤﭙﻠﮑﺲﻫﺎي ﮔﻮﻧﻪاي
Title of article :
The Iranian Hyalomma (Acari: Ixodidae) with molecular evidences to understand taxonomic status of species complexes
Author/Authors :
Hosseini-Chegeni, Asadollah Department of Plant Protection - Pol-e Dokhtar Higher Education Center - Lorestan University , Hosseini, Reza Department of Plant Protection - Faculty of Agriculture - University of Guilan , Telmadarraiy, Zakkyeh Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences , Abdigoudarzi, Mohammad Razi Vaccine and Serum Research Institute - Department of Parasitology - Reference Laboratory of Ticks and Tick Borne Diseases
Abstract :
The identification of Hyalomma is a challenging issue in the systematics of ixodid ticks. Here, we examined 960 adult males of Hyalomma tick from 10 provinces of Iran using morphological and molecular methods. PCR was carried out on 60 samples to amplify an ITS2 fragment of nuclear and a COI fragment of mitochondrial genomes. Nine species, namely H. aegyptium, H. anatolicum, H. asiaticum, H. scupense, H. dromedarii, H. excavatum, H. marginatum, H. rufipes and H. schulzei were identified. The validity of H. rufipes and H. excavatum can be challenged. We concluded that these species should be regarded as H. marginatum and H. anatolicum complexes, respectively. Furthermore, the taxonomic status of the two closely related H. anatolicum and H. asiaticum was confirmed as distinct species.
Farsi abstract :
ﭼﮑﯿﺪه ﺷﻨﺎﺳﺎﯾﯽ ﮐﻨﻪﻫﺎي Hyalomma ﻣﻮﺿﻮﻋﯽ ﭼﺎﻟﺶ ﺑﺮاﻧﮕﯿﺰ در ﺳﯿﺴﺘﻤﺎﺗﯿﮏ ﮐﻨﻪﻫﺎي ﺳﺨﺖ اﺳﺖ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، 960 ﮐﻨﻪ ﻧﺮ ﺑﺎﻟﻎ Hyalomma از 10 اﺳﺘﺎن در ﮐﺸﻮر اﯾﺮان را ﺑﺎ روشﻫﺎي رﯾﺨﺖﺷﻨﺎﺳﯽ و ﻣﻮﻟﮑﻮﻟﯽ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار داده ﺷﺪﻧﺪ. PCR روي 60 ﻧﻤﻮﻧﻪ ﺑﻪ ﻣﻨﻈﻮر ﺗﮑﺜﯿﺮ ﺑﺨﺸﯽ از ژن ITS2 ﻫﺴﺘﻪاي و ﺑﺨﺸﯽ از ژن COI ﻣﯿﺘﻮﮐﻨﺪرﯾﺎﯾﯽ ژﻧﻮم اﻧﺠﺎم ﮔﺮﻓﺖ. ﻧُﻪ ﮔﻮﻧﻪ ﺷﺎﻣﻞ H. asiaticum ،H. anatolicum ،H. aegyptium، H. ﺷﻨﺎﺳﺎﯾﯽ ﺷﺪﻧﺪ. ﻫﻮﯾﺖ دو ﮔﻮﻧﻪ H. schulzei و H. rufipes ،H. marginatum ،H. excavatum ،H. dromedarii ،H. scupense H. marginatum ﺑﺎ ﭼﺎﻟﺶ ﻫﻤﺮاه اﺳﺖ. ﺑﺮ اﺳﺎس ﻧﺘﺎﯾﺞ اﯾﻦ ﭘﮋوﻫﺶ، اﯾﻦ ﮔﻮﻧﻪﻫﺎ ﺑﺎﯾﺴﺘﯽ ﺑﻪ ﺗﺮﺗﯿﺐ ﻫﻤﺎن دو ﮔﻮﻧﻪ H. excavatum و rufipes و H. anatolicum در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﻮﻧﺪ. ﻫﻤﭽﻨﯿﻦ وﺿﻌﯿﺖ ﺗﺎﮐﺴﻮﻧﻮﻣﯿﮏ دو ﮔﻮﻧﻪ ﻧﺰدﯾﮏ ﺑﻪ ﻫﻢ H. anatolicum و H. asiaticum ﺑﻪ ﻋﻨﻮان ﮔﻮﻧﻪﻫﺎي ﻣﺴﺘﻘﻞ ﺗﺄﯾﯿﺪ ﺷﺪ.
Keywords :
Hyalomma , phylogenetic relationships , taxonomy , variation , Iran
