Author/Authors :
Fallah, Zari School of Chemistry - Shahrood University of Technology, Shahrood, Iran , Nasr Isfahani, Hossein School of Chemistry - Shahrood University of Technology, Shahrood, Iran , Tajbakhsh, Mahmood Faculty of Chemistry - University of Mazandaran, Babolsar, Iran , Mohseni, Mojtaba School of Molecular and Cell Biology - University of Mazandaran, Babolsar, Iran , Tashakkorian, Hamed Cellular and Molecular Biology Research Center - Health Research Institute - Babol University of Medical Sciences, Babol, Iran , Amouei, Abdoliman Environmental Health Engineering Department - Babol University of Medical Sciences, Babol, Iran , Zabihi, Ebrahim Department of Pharmacology - School of Medicine - Babol University of Medical Sciences, Babol, Iran , Abedian, Zeinab Cellular and Molecular Biology Research Center - Health Research Institute - Babol University of Medical Sciences, Babol, Iran
Abstract :
TiO2-grafted cellulose bio-nanocomposite (Cell.Com) was successfully prepared by combining
modified cellulose and TiO2 nanoparticles via copper-catalyzed alkyne-azide click reaction. The
antibacterial and cytotoxicity effects of Cell.Com containing cellulose, triazole ring and TiO2
moieties are reported in this work. The antibacterial activity of Cell.Com was investigated against
gram positive (S. aureus and B. subtilis) and gram negative (E. coli and P. aeruginosa) bacteria. The
antibacterial results indicate that the Cell.Com exhibits a wide range of antibacterial activity against
both gram positive and gram negative bacteria which is due to the presence of TiO2 and triazole
ring moieties in its structure. In addition, the cytotoxicity effect of Cell.Com was studied in vitro on
cervical cancer (HeLa), breast carcinoma (MCF-7), sarcoma osteogenic (Saos) and fibroblast cells
by MTT assay. The cell viability results show that the Cell. Com diminishes relatively cancer cells
growth in a dosage and time dependent way probably through triazole moiety, while no apparent
cytotoxicity effect is observed on the normal fibroblast cells.
Keywords :
Cytotoxicity , Antibacterial , TiO2 , Cellulose , Nanocomposite
