Title of article :
Expression of estrogenic response genes to different concentration of 17ß-estradiol in male mosquitofish (Gambusia yucatana)
Author/Authors :
Rendón von Osten, J Instituto de Ecología - Pesquerías y Oceanografía del Golfo de México - Universidad Autónoma de Campeche - San Francisco de Campeche - Campeche, México , Aguayo-Dionet, G Instituto de Ecología - Pesquerías y Oceanografía del Golfo de México - Universidad Autónoma de Campeche - San Francisco de Campeche - Campeche, México , dzul-Caamal, R Instituto de Ecología - Pesquerías y Oceanografía del Golfo de México - Universidad Autónoma de Campeche - San Francisco de Campeche - Campeche, México , Lara- Flores, M Instituto de Ecología - Pesquerías y Oceanografía del Golfo de México - Universidad Autónoma de Campeche - San Francisco de Campeche - Campeche, México
Abstract :
The estrogenic effects of endocrine disrupting compounds in fish are not reversible and can reduce populations. Sensitive methods such as Q-PCR, Western blot, microarrays, have been used to determine changes in gene expression and this predict the effects before they become irreversible. The present study was designed to detect the expression of the estrogen receptors vitellogenin and pregnane X indicates that they are potentially useful molecular markers for detecting the presence of endocrine disrupting compounds in the environment. The results demonstrate that the exposure to the hormone 17ß-estradiol induced the expression of estrogenic response genes in male mosquito fish (G. yucatana) and this is related to the alterations to the endocrine system caused by the hormone.
Keywords :
Gene expression , Biomarkers , Endocrine disrupting compounds (EDCs) , Mosquitofish (Gambusia yucatana) , vitellogenin , Q-PCR
