Author/Authors :
Kim, J.H Department of Laboratory Animal Medicine - College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea , Li,L Institute of Medical Science - Kangwon National University, Chuncheon, Republic of Korea , Park, D.H Department of Oriental Medicine Material - Dongsin University, Republic of Korea , Lee, I.W Department of Laboratory Animal Medicine - College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea , Ham, N Department of Laboratory Animal Medicine - College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea , Kim, H.S Radiation Health Research Institute - Korea Hydro and Nuclear Power Co.,Ltd. Seoul, Republic of Korea , Kim, Y.J Korea Food Research Institute, Seongnam, Kyeonggi, Republic of Korea , Shim, J Cancer Experimental Resources Branch - National Cancer Center, Republic of Korea , Kwon, S.W CHA Bundang Medical Center - CHA University, Bundang-gu, Seongnam-si, Republic of Korea , Lee, M.J Department of Laboratory Animal Medicine - College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea
Abstract :
Background: People are exposed to more radiation than before with the
application of radiation technology. Radiation is known to induce damage to cell
structure, DNA, chromosomes and nucleus. In this study, we showed that CGJ
extract can inhibit radiation-induced chromosomal damage in vivo and NLRP7
inflammasome activation in vitro, suggesting that the compound from CGJ can Be
considered as a therapeutic materials to reduce adverse effect inflammation and
chromosome aberration during radio-therapy. Materials and methods: In this study
the inhibitory effects of Cheonggukjang (CGJ) extract on the radiation-induced
micronucleus formation in vivo and the NOD-like receptor family pyrin
domain-containing 3 (NLRP3) inflammasome activation in vitro. Results: We
observed the prolonged presence of radiation-induced inflammation and its
onsecutive damage in hematopoietic tissues after irradiation by examining
micronucleus formation in polychromatic erythrocytes. Mouse bone marrowderived
macrophages were used to investigate NLRP3 inflammasome activation.
The micronucleus analysis with mouse peripheral polychromatic erythrocytes using
acridine orange staining showed a significantly (p<0.05) reduced frequency of
micronucleated bone marrow polychromatic erythrocytes (MnPCEs) in CGJ-treated
mice. Conclusion: CGJ extract elevated the chance that mice would survive a
potentially lethal dose of gamma-irradiation. Additionally, The CGJ extract
attenuated IL-1β maturation by interrupting the inflammasome. The CGJ extract
attenuates radiation-induced micronucleus formation possibly mediated by
inhibiting inflammasome, which provides valuable information for the further study
of the mechanism of action of CGJ extract.
Keywords :
NLRP3 , inflammasome , micronuclei , radiation , Cheonggukjang