Author/Authors :
Yasin, Salem R Department of Biology and Biotechnology - Faculty of Science - the Hashemite University, Zarqa, Jordan , AlHawari, Hussam H Department of Internal Medicine - Faculty ofMedicine - University of Jordan,Amman,Jordan , Alassaf, Abeer A Department of Pediatric - Faculty of Medicine - University of Jordan, Amman, Jordan , Khadra, Maysa M Department of Obstetrics and Gynecology - Faculty of Medicine - University of Jordan, Amman, Jordan , Al-Mazaydeh, Zainab A Department of Biology and Biotechnology - Faculty of Science - the Hashemite University, Zarqa, Jordan , Al-Emerieen, Ala'a F Department of Service Courses - Faculty of Science - Zarqa Private University, Zarqa, Jordan , Tahtamouni, Lubna H Department of Biology and Biotechnology - Faculty of Science - the Hashemite University, Zarqa, Jordan
Abstract :
Background:Hypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimedto address the polymorphic nature of AluDNA fragmentin the human tissue plasminogen activator gene within diabetes mellitus(DM) Jordanianpatients.Methods:Genomic DNA was isolated from 76 DM patients and 60 non-diabetic Jordanian individuals, and the Alu fragment was amplified using PCR. Results: The results showed that 80% of the non-diabeticJordaniansubjectswere homozygotes for the deletionof the Alufragment(Alu-/-),16.7% were homozygotes for itsinsertion(Alu+/+),and 3.3% were heterozygotes (Alu+/-).Besides, 36.8% of the diabetic patients exhibited the Alu-/-or Alu+/-genotype, and 26.3% were Alu+/+.The Alu-/-genotype occurred less frequently in the diabeticindividuals. Conclusion:The high frequencyof the Alu-/-genotype constitutesa protective deletion with respect to DMwithin the normal subjects.
