Title of article :
Molecular Rapid Diagnostic Test for Nosocomial Nonfermenting Gram-Negative Bacilli
Author/Authors :
Emami, Amir Microbiology Department - Shiraz University of Medical Sciences , Pirbonyeh, Neda Microbiology Department - Shiraz University of Medical Sciences , Bazargani, Abdollah Department of Bacteriology and Virology - School of Medicine - Shiraz University of Medical Science , Javanmardi, Fatemeh Microbiology Department - Shiraz University of Medical Sciences
Abstract :
Accurate diagnosis of nosocomial infections is crucial for appropriate antibiotic therapy, as well as avoiding unnecessary use of drugs. Mainly 3 gram-negative bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are involved in nosocomial infections. These bacteria are possibly misdiagnosed with together in traditional diagnostic methods. In this regard, a rapid molecular diagnostic kit was developed in Shiraz Burn Research Center. Using this diagnostic kit, the sensitivity and specificity analyses showed following results, respectively: P. aeruginosa: 100%, 96.8%; A. baumannii: 100%, 100%, and S. maltophilia: 96.7%, and 93.8%. Therefore, this multiplex polymerase chain reaction (PCR) method with 3 primer sequences could be a responsive technique in timely and appropriate detection of studied bacteria.
Keywords :
Pseudomonas aeruginosa , Acinetobacter baumannii , Stenotrophomonas maltophilia , Nosocomial infection
Journal title :
Avicenna Journal of Clinical Microbiology and Infection
