Title of article :
Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae
Author/Authors :
Mirzaei Seveiri ، Rasool Applied Microbiology Research Center, Systems Biology and Poisonings Institute - Baqiyatallah University of Medical Sciences , Hamidi ، Masoud Department of Medical Biotechnology - Faculty of Paramedicine, Food and Drug Research Center, Vice-Chancellery of Food and Drug - Guilan University of Medical Sciences , Delattre ، Cédric CNRS, SIGMA Clermont, Institut Pascal - Université Clermont Auvergne , Sedighian ، Hamid Applied Microbiology Research Center, Systems Biology and Poisonings Institute - Baqiyatallah University of Medical Sciences , Pierre ، Guillaume CNRS, SIGMA Clermont, Institut Pascal - Université Clermont Auvergne , Rahmani ، Babak Department of Molecular Medicine - Faculty of Medicine - Qazvin University of Medical Sciences , Darzi ، Sina Department of Molecular Medicine - School of Advanced Technologies - Shahrekord University of Medical Sciences , Brasselet ، Clément CNRS, SIGMA Clermont, Institut Pascal - Université Clermont Auvergne , Karimitabar ، Fatemeh Food and Drug Research Center, Vice-Chancellery of Food and Drug - Guilan University of Medical Sciences , Razaghpoor ، Ali Student Research Committee, Nursing and Midwifery Faculty - Qazvin University of Medical Sciences , Amani ، Jafar Applied Microbiology Research Center, Systems Biology and Poisonings Institute - Baqiyatallah University of Medical Sciences
Abstract :
Purpose: Due to the potential industrial and therapeutic applications of the yeast exopolysaccharides (EPSs), there has been an increasing demand to assess these biopolymers with improved characteristics. This study aimed to characterize the EPSs from Rhodosporidium babjevae (ATCC 90942 and IBRC-M 30088) as well as to evaluate their possible antioxidant, emulsifying and antiproliferative activities.Methods:Rhodosporidium babjevae was cultured for 5 days and following isolation of supernatant, EPSs precipitated with adding of cold absolute ethanol and freeze-dried. The EPSs chemical structure was determined by FT-IR, SEM, HPLC-SEC and GC-MS. Additionally the solubility, water holding capacity and emulsifying activity of EPSs were evaluated. In vitro, antioxidant activity was investigated against DPPH, superoxide and hydroxyl radicals. Finally the EPSs consequence on the cell proliferation of human breast adenocarcinoma (MCF-7) and Madin-Darby canine kidney (MDCK) cell lines was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test.Results:R. babjevae excreted 1.6±0.2 g/L of the EPSs. The EPSs had three fractions with molecular weights of 1.02 ×10^6, 5×10^5 and 2×10^5 Da. Mannose and glucose were found as the main monosaccharides of the EPSs (84:16 mol%, respectively). The EPSs exhibited emulsifying activity on sun flower oil. The scavenging activities were found to be dose-dependent and higher than hyaluronic acid.Significant difference among the EPSs treatments on the proliferation of MCF-7 and MDCK cell lines was not observed (P 0.05). Conclusion: These results show the interesting potential of the EPSs from R. babjevae as biocompatible compounds for using in food and pharmaceutical fields.
Keywords :
Exopolysaccharides , Rhodosporidium babjevae , Antioxidant activity , Emulsifying activity , Antiproliferative activity
Journal title :
Advanced Pharmaceutical Bulletin
