Title of article :
An evaluation of frequencies and types of bacterial pathogens in personnel gowns, before and after washing with sodium hypochlorite 5%, in Vali-e-Asr Hospital, Birjand, Iran
Author/Authors :
Maleki Asma Faculty of Paramedicine - Tehran University of Medical Sciences - Tehran, Iran , Shamshirgaran Marzieh Faculty of Paramedicine - Birjand University of Medical Sciences - Birjand, Iran , Aramjoo Hamed Faculty of Paramedicine - Birjand University of Medical Sciences - Birjand, Iran , HajiHosseini Morteza Alberta University - Edmonton, Canada , Malaki Moghadam Hakimeh Clinical Research Development Unit of Vali-e-Asr Hospital - Birjand, Iran , Zare Bidaki Majid Infectious Disease Research Center - Birjand University of Medical Sciences - Birjand, Iran
Abstract :
Microbial contamination of hospital gowns is the main reason for the dissemination of nosocomial
infections. Bacteria are the cause of most of these contaminations. The present study aimed at determining the
frequencies and types of bacterial pathogens on the surface of personnel gowns, before and after washing with sodium
hypochlorite 5% in Vali-e-Asr Hospital, Birjand, Iran.
Methods: In the current study, the sampling was carried out in two steps, before and after washing with sodium
hypochlorite. Sampling was performed with a sterile swap in the transport medium. Samples were incubated for 3 h and
were cultured on blood agar and eosin methylene blue agar. After overnight incubation and colonization, the
identification was conducted using macroscopic, microscopic, rapid enzymatic, and biochemical tests.
Results: The most common bacterium before washing was Pseudomonas aeruginosa and the least common was
Enterobacter cloacae. It was revealed that sodium hypochlorite exert the most profound effect on Pseudomonas
aeruginosa, whereas the least effect was detected on Staphylococcus aureus and Staphylococcus epidermidis.
Conclusions: The type of bacteria involved in nosocomial infections could be different among hospitals. Sodium
hypochlorite 5% is recommended for washing hospital gowns to reduce bacterial contamination.
Keywords :
Sodium hypochlorite , Nosocomial infections , Disinfection
Journal title :
Journal of Surgery and Trauma