FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection

background: brucella spp. are grampositive, rodshaped, and sporeforming bacilli. brucella abortus and b. melitensis are the main causes of brucellosis. objectives:the aim of the study was to establish a rapid and simple molecular method for the detection of this disease. methods:fortyfive brucella spp. were isolated from blood samples using the bactec fluorescent 9050 system and were detected by antiigm or igg brucella specific antigen. dna extraction was conducted on all samples. fluorescent amplification based specific hybridization (flashpcr) test was utilized to detect the 351bp fragment from eryd gene, which was specific for brucella spp. results:a 351bp fragment resulted from pcr reaction and showed the accuracy of designed primers. this fragment was successfully amplified in the flashpcr reaction. in this study, we have positive and negative samples and a standard method. in addition, we calculated the sensitivity and specificity of this method as 100%. conclusions:results of the study was proved that the flashpcr method was a rapid, sensitive, and safe method for the detection of brucella genome in whole blood samples of patient harbored brucellosis and is recommended for routine usage.