Title of article :
Detection of Common Respiratory Viruses in Patients with Acute Respiratory Infections Using Multiplex Real-Time RT-PCR
Author/Authors :
neisi, niloofar Infectious and Tropical Diseases Research Center - Health Research Institute - Ahvaz Jundishapur University of Medical Sciences , abbasi, samaneh Abadan Faculty of Medical Sciences , makvandi, manoochehr Infectious and Tropical Diseases Research Center - Health Research Institute - Ahvaz Jundishapur University of Medical Sciences , salmanzadeh, shokrollah Infectious and Tropical Diseases Research Center - Health Research Institute - Ahvaz Jundishapur University of Medical Sciences , biparva, somayeh Department of General Courses - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , nahidsamiei, rahil Infectious and Tropical Diseases Research Center - Health Research Institute - Ahvaz Jundishapur University of Medical Sciences , varnaseri ghandali, mehran Deputy of Health - Ahvaz Jundishapur University of Medical Sciences , rasti, mojtaba Infectious and Tropical Diseases Research Center - Health Research Institute - Ahvaz Jundishapur University of Medical Sciences , ahmadi angali, kambiz Biostatistics Department - School of Health - Ahvaz Jundishapur University of Medical Sciences
Pages :
6
From page :
1
To page :
6
Abstract :
background: acute respiratory infection (ari) is caused by human metapneumovirus (hmpv), respiratory syncytial virus type a and b (rsv-a, rsv-b), human parainfluenza viruses 1, 2, and 3 (hpiv-1, hpiv-2, and hpiv-3), influenza viruses a and b (ifv-a, ifv-b), and human coronaviruses (oc43/hku1, nl63, 229e) worldwide. objectives: this study was conducted to assess the causative agents of viral ari among hospitalized adults by real-time pcr. methods: clinical nasopharyngeal swabs of 112 patients including 55 (49.1%) males and 57 (50.89%) females with ari were analyzed using multiplex real-time rt-pcr. results: out of 112 specimens, 61 (54.46%) were positive including 10 (8.9%) for influenza h3n2, one (0.89%) for influenza b, 28 (25%) for rsv-a, 18 (16.07%) for hmpv-a, two (1.78%) for hpiv-1, and three (2.67%) for hpiv-3. two (1.78%) specimens were positive for two agents, rsv-a/hmpv-a and rsv-a/hpiv-3. the distribution of viral infections was 30 among males (26.78%) and 31 (27.67%) among females (p = 0.862). high frequency of rsv-a infection (25%) and the low frequency of influenza b virus infection (0.89%) were detected among patients. the remaining 51 (45.53%) samples were negative for rsv-b, hmpv-b, ifv-a, hpiv2-4a-4b, and hcovs (oc43/hku1, nl63, 229e). conclusions: the role of other viruses such as human adenoviruses rhinovirus/enterovirus (rv/ev), human bocavirus (hbov), and human parechovirus (hpev) was not investigated. multiplex pcr can be used as a rapid test for the diagnosis of viruses causing acute respiratory infection, which results in decreased length of hospitalization.
Keywords :
Acute Respiratory Infection , Real-Time Polymerase Chain Reaction , Genotype
Journal title :
Jundishapur Journal of Microbiology (JJM)
Serial Year :
2019
Record number :
2500409
Link To Document :
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