The Prevalence of Atypical Pneumonia Caused by Mycoplasma pneumoniae (P1 gene) in Patients with Respiratory Infections by Culture and Molecular PCR Methods in Tehran, Iran

background:atypical pneumonia is an upper and lower respiratory tract infection. mycoplasma pneumoniae is a major cause of communityacquired pneumonia (cap).objectives:the present study aimed to determine the prevalence of atypical pneumonia caused by m. pneumonia by culture and molecular pcr methods in tehran.methods:in the present study, 102 samples of throat swab were collected from patients with respiratory infections. all samples were cultured in liquid pplo broth and solid pplo agar media (1% glucose and 20% horse serum). the pcr technique with specific primers was implemented after culture and genome extraction through phenolchloroform technique.results:in this study, 27 (26.47%) colonies of mycoplasma were isolated on pplo agar medium. using specific primers, it was found that 33 samples (32.4%) were positive in terms of mycoplasma genus and 14 samples (13.7%) were positive for the presence of m. pneumonia.conclusions:mycoplasma pneumonia is a pathogen that causes respiratory tract infections in humans. molecular pcr method is a quick and sensitive technique that has higher sensitivity and specificity than other methods. the obtained results may contribute to the specific treatment of some patients with symptoms of respiratory infections.