Title of article :
Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues
Author/Authors :
Baradaran Ghavami, Shaghayegh Gastroenterology and Liver Diseases Research Center - Shahid Beheshti University of Medical Sciences , Kabiri, Fateme Gastroenterology and Liver Diseases Research Center - Shahid Beheshti University of Medical Sciences , Nourian, Mahyar Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Shahid Beheshti University of Medical Sciences , Balaii, Hedieh Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Shahid Beheshti University of Medical Sciences , Shahrokh, Shabnam Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Shahid Beheshti University of Medical Sciences , Chaleshi, Vahid Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Shahid Beheshti University of Medical Sciences , Sherkat, Ghazal Student Research Committee - Islamic Azad University - Mashhad Branch , Shalileh, Farzaneh Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Shahid Beheshti University of Medical Sciences , Asadzadeh Aghdaei, Hamid Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Shahid Beheshti University of Medical Sciences
Abstract :
Aim: In the present study, two main variants of ATG16L1 gene, rs2241880 T300A and rs2241879 C/T, were evaluated in IBD patients as well as in remission and flareup phase across an Iranian population for the first time.
Background: Inflammatory bowel disease (IBD) has found increasing global incidence and prevalence in recent years especially among pediatrics. ATG16L1 is the major gene that regulates autophagy pathway. The autophagy pathway also affects dysbiosis.
Methods: Genomic DNA was isolated from peripheral blood samples following salting out extraction method. The genotypes of ATG16L1 polymorphisms rs2241880 T300A and rs2241879 C/T were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Results: In this case control study, a total of 101 IBD patients (75 ulcerative colitis (UC) and 26 Crohn’s disease (CD)) and 99 healthy controls were evaluated. In the present study, a significant association was found between rs2241879 single nucleotide polymorphism on ATG16L1 gene and increased risk of IBD among an Iranian population (P=0.01). There was no statistically significant relationship between rs2241880 and IBD risk (P= 0.42). The effect on these two variants was investigated in relapse and flareup phase which was not significant either, but in CD, rs2241879 and rs2241880 were difference in the relapse phase.
Conclusion: The results showed that ATG16L1 gene rs2241879 has a significant relationship with increased risk of IBD among an Iranian population. Individuals with C allele showed a significant relationship with 1.68-fold increased risk of IBD (P=0.01; adjusted OR=1.68; 95% CI=1.13-2.50).
Keywords: Autophagy, ATG16L1, Inflammatory bowel disease, Diseases status.
(Please cite as: Baradaran Ghavami SH, Kabiri F, Nourian M, Balaii H, Shahrokh SH, Chaleshi V, et al. Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues. Gastroenterol Hepatol Bed Bench 2019;12(Suppl.1):S94-S100).
Keywords :
Autophagy , ATG16L1 , Inflammatory bowel disease , Diseases status
Journal title :
Gastroenterology and Hepatology From Bed to Bench
