Author/Authors :
Nilforoushzadeh, Mohammad Ali Skin and Stem Cell Research Center -Tehran University of Medical Sciences , Kazemikhoo, Nooshafarin Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Department of Dermatology - St. George Hospital - UNSW - Sydney, Australia , Mokmeli, Soheila Canadian Optic and Laser Centre - COL Centre - BC, Canada , Zare, Sona Department of Biology - Faculty of Science - Islamic Azad University - Hamedan Branch, Hamedan - Skin and Stem Cell Research Center -Tehran University of Medical Sciences , Dahmardehei, Mostafa Burn Research Centre - Department of Plastic and Reconstructive Surgery - Iran University of Medical Sciences, Tehran , Vaghar Doost, Reza Burn Research Centre - Department of Plastic and Reconstructive Surgery - Iran University of Medical Sciences, Tehran , Momeni, Mahnoush Burn Research Centre - Department of Plastic and Reconstructive Surgery - Iran University of Medical Sciences, Tehran , Ansari, Fereshteh Research Centre for Evidence-Based Medicine - Health Management and Safety Promotion Research Institute - Tabriz University of Medical Sciences, Tabriz - Iranian EBM Centre: A Joanna Briggs Institute Affiliated Group
Abstract :
Introduction: Low-level laser therapy (LLLT) has been used as an effective therapeutic modality since the mid-1960s. Although there have been several clinical studies using LLLT in wound healing, especially diabetic, pressure and venous ulcers, there are few reports of using this technique in burn ulcers. Autologous fibroblast transplantation is a novel treatment for patients with burns or venous ulcers. In this study for the first time, we used LLLT along with autologous fibroblast skin transplantation to treat grade 3 burn ulcers in diabetic patients. This case series describes the successful management of grade 3 burn ulcers in 10 diabetic patients using autologous fibroblast transplantation along with LLLT.
Methods: After the approval of the Tehran University Ethics Committee (IR.TUMS.REC.1394.1683) and the Iran Registry of Clinical Trials (IRCT2016050226069N3), 10 diabetic patients with 10 grade 3 burn ulcers, who were a candidate for skin graft surgery, entered the study. Donor skin was biopsied using a 3 mm punch. Fibroblasts were extracted and cultured in vitro in the GMP Technique laboratory. The patients were treated using LLLT in 3-4 weeks during the time that fibroblast cultures became ready to use. Laser irradiation was done using red light, 650 nm, 150 mW, 1 J/cm2 for the bed of the ulcer and infra-red light 808 nm, 200 mW, 6 J/cm2 for the margins every other day for 10 sessions.
Results: The mean wound size before treatment was 16.28 cm2. All patients’ burn wounds healed completely after 10-12 weeks.
Conclusion: We conclude that this method can be used as an effective method for treating large wounds, especially in complicated patients including the diabetics.
