Title of article :
Sponge-associated fungi from a mangrove habitat in Indonesia: species composition, antimicrobial activity, enzyme screening and bioactive profiling
Author/Authors :
Sibero, Mada Triandala Department of Marine Science - Diponegoro University, Semarang, Indonesia , Radjasa, Ocky Karna Department of Marine Science - Diponegoro University, Semarang, Indonesia , Sabdono, Agus Department of Marine Science - Diponegoro University, Semarang, Indonesia , Trianto, Agus Department of Marine Science - Diponegoro University, Semarang, Indonesia , Igarashi, Yasuhiro Department of Biotechnology - Biotechnology Research Center - Toyama Prefectural University, Imizu, Toyama, Japan , Zilda, Dewi Seswita Research and Development Center for Marine, Jakarta, Indonesia , Wijaya, Yusuf Jati Department of Oceanography - Diponegoro University, Semarang, Indonesia
Pages :
14
From page :
173
To page :
186
Abstract :
There is no report of diversity, biological properties and bioactive compounds of sponge-associated fungi from Indonesia’s mangrove ecosystem to date. This study was designed to isolate sponge-associated fungi from a mangrove ecosystem in Mangkang, to screen the antimicrobial and extracellular enzyme properties of the isolates, characterize the biologically promising isolates using molecular approaches, and profile the secondary metabolites using phytochemical and thin-layer chromatography (TLC) analyses. An unidentified sponge that lived in association with mangrove roots was collected from Mangkang. Total of eight associated fungi were isolated from the sponge. Among all isolates, only two fungi SPMKF 1 and SPMKF 6 produced extracellular amylase, another two fungi SPMKF 4 and 5 showed antibacterial activity against MRSA, and only one fungus SPMKF 8 was able to produce extracellular amylase and show antimicrobial activity against ESBL E. coli, Salmonella enterica ser. Typhi strain MDR and C. albicans, while SPMKF 2, SPMKF 3 and SPMKF 9 did not show any biological properties. The result of genetic characterization proved that SPMKF 1 was Cladosporium tenuissimum, SPMKF 4 was Eutypella sp., SPMKF 5 was Lasiodiplodia theobromae, SPMKF 6 was Fusarium keratoplasticum and SPMKF 8 was F. solani. Furthermore, an amylase gene was detected in fungi SPMKF 1, 6 and 8 while among the BGC, only NRPS genes were detected in SPMKF 4, 5 and 8. Interestingly, several same metabolites indicating the same retention factor (Rf) values in TLC were detected in the fungal crude extracts by TLC.
Keywords :
Fungi , NRPS , Sponge , Mangrove ecosystem
Journal title :
International Aquatic Research
Serial Year :
2019
Record number :
2501603
Link To Document :
بازگشت