Title of article :
Purification of PIA and rSesC as Putative Vaccine Candidates Against Staphylococcus aureus
Author/Authors :
Bahonar, Sara Department of Medical Microbiology and Virology - Faculty of Medicine - Mazandaran University of Medical Science , Ghazvinian, Maryam Department of Medical Microbiology and Virology - Faculty of Medicine - Mazandaran University of Medical Science , Haghshenas, Mohamad Reza Department of Medical Microbiology and Virology - Faculty of Medicine - Mazandaran University of Medical Science , Goli, Hamid Reza Department of Medical Microbiology and Virology - Faculty of Medicine - Mazandaran University of Medical Science , Mirzaei, Bahman Department of Medical Microbiology and Virology - Faculty of Medicine - Mazandaran University of Medical Science
Abstract :
Background: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and
frequently infects hospitalized individuals.
Methods: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major
macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a
prokaryotic host.
Results:Purification of the macromolecules was confirmed by FTIR and Western blotting.
Conclusions: The S. epidermidis SesC protein and PIA were successfully purified. Both are considered as
vaccine candidates.
Keywords :
Biofilm formation , Polysaccharide Intracellular Adhesin , Staphylococcus aureus
Journal title :
Reports of Biochemistry and Molecular Biology (RBMB)
