Author/Authors :
Mehrvar, Narjes Cancer Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Abolghasemi, Hassan Pediatric Congenital Hematologic Disorders Research Center - Research Institute for Children's Health - Shahid Beheshti University of Medical , Tehran, Iran , Rezvany, Mohammad Reza Hematology department - Faculty of Allied Medicine - Iran University of Medical Sciences, Tehran, Iran , Akbari, Mohammad Esmaeil Cancer Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Saberynejad, Javad AJA University of Medical Sciences, Tehran, Iran , Mehrvar, Azim AJA University of Medical Sciences, Tehran, Iran , Ehsani, Mohammad Ali Bahrami Hospital - Tehran University of Medical Sciences, Iran , Nourian, Mahyar Mahak Hematology Oncology Research Center (Mahak-HORC) - Mahak Hospital, Tehran, Iran , Qaddoumi, Ibrahim Department of Global Pediatric Medicine - St. Jude Children’s Research Hospital- Memphis, TN USA , Movafagh, Abolfazl Department of Medical Genetics - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Background:Abnormal expression of ABCC transporter genes has been associated with treatment failure in
pediatric patients with acute lymphoblastic leukemia (ALL). The aim of this study was to evaluate the
expression pattern of ABCC1-6 and ABCC10 genes in Iranian pediatric patients with ALL relapse and
determine the potential predictive value of determining ALL relapse from ABCC expression.
Methods:Patients with ALL were divided into two separate groups, either the case group with relapsed ALL
or the control group in which ALL patients have been in progression-free survival for at least 3 years A total
of thirty-nine participants (23 with relapsed ALL; 16 controls) were enrolled over 26 months. To determine
the levels of ABCC1-6 and ABCC10 transporter gene expression RT-PCR was used. Cumulative doses of the
chemotherapy drugs, VCR, DNR and L-ASP, were calculated for each patient.
Results:Our findings showed elevated expression of ABCC2-6 and decreased expression of ABCC1 and
ABCC10 to be associated with an increased risk of ALL relapse. The mean-fold expression of ABCC2 was
significantly increased in the ALL relapse group. Additionally, the expression pattern of the ABCC transporter
genes was associated with high doses of three chemotherapy drugs, VCR, DNR and L-ASP.
Conclusions:Evaluating the expression pattern of ABCC transporter genes may be a potential biomarker for
predicting the occurrence of ALL relapse in Iranian pediatric patients and improve cancer prognosis.
Keywords :
ABC transporters , Childhood , Gene expression , Pattern , Recurrence
