Author/Authors :
Nadimi, Hoda Department of Cellular and Molecular Nutrition - School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Iran , Djazayery, Abolghassem Department of Community Nutrition - School of Nutritional Sciencesand Dietetics - Tehran University of Medical Sciences, Iran , Javanbakht, Mohammad Hassan Department of Cellular and Molecular Nutrition - School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Iran , Dehpour, Ahmadreza Experimental Medicine Research Center - Tehran University of Medical Sciences, Iran , Ghaedi, Ehsan Department of Cellular and Molecular Nutrition - School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Iran , Derakhshanian, Hoda Department of Biochemistry, Nutrition and Genetics - Medical School, Alborz University of Medical Sciences, Karaj, Iran , Mohammadi, Hamed Student Research Committee - Department of Clinical Nutrition - School of Nutrition and Food Science - Isfahan University of Medical Sciences, Iran , Zarei, Mahnaz Department of Cellular and Molecular Nutrition - School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Iran , Djalali, Mahmoud Department of Cellular and Molecular Nutrition - School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Iran
Abstract :
Background:Diabetes mellitus and metabolic disorders are a major burden on the healthcare system. Irisin is
a novel myokine reported to have beneficial effects on glucose and lipid metabolism. Vitamin D deficiency
has been implicated in the development of diabetes and hold a critical role in diabetes-related complications.
In the present study, we examined the efficacy of vitamin D supplementation on serum irisin levels, skeletal
muscle irisin levels, and the expression of the irisin precursor, FNDC5 (fibronectin-type III domain-containing
5) in type I diabetes mellitus rats.
Methods: Thirty-six adult male Sprague-Dawley rats (150 – 250 g) were randomly divided into four groups:
group I: healthy control rats with no treatment (n=8), group II: healthy control rats receiving sesame oil as a
placebo (n=8), group III: diabetic rats receiving sesame oil as placebo (n=10), group IV: diabetic rats treated
with 4300 IU/kg/week vitamin D (n=10). Diabetes was induced by intraperitoneal (IP) injection of
streptozotocin. At the end of the vitamin D intervention blood and triceps muscle samples were collected.
RNA was extracted from muscle and real-time PCR was performed to examine FNDC5 gene expression.
Results:Our study showed that the administration of vitamin D (4300 IU/kg/week) in a streptozotocin-diabetic rat
model resulted in increased serum vitamin D levels, FNDC5 gene expression and muscle irisin levels. However,
the levels of serum irisin were not significantly changed by the administration of vitamin D.
Conclusions: In conclusion, we show that vitamin D supplementation enhances serum vitamin D levels,
FDNC5 gene expression and muscle irisin levels in the streptozotocin-diabetic rat model. Our study highlights
the potential therapeutic effect of vitamin D supplementation for diabetes mellitus.
