Title of article :
Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice
Author/Authors :
Zangeneh, Mohammad Mahdi Department of Clinical Science - Faculty of Veterinary Medicine - Razi University, Kermanshah, Iran , Zangeneh, Akram Department of Clinical Science - Faculty of Veterinary Medicine - Razi University, Kermanshah, Iran , Tahvilian, Reza Pharmaceutical Sciences Research Center - Health Institute -Kermanshah University of Medical Sciences, Iran , Moradi, Rohallah Biotechnology and Medicinal Plants Research Center - Ilam University of Medical Sciences, Iran
Abstract :
Background and objectives: Tragopogon graminifolius DC. is widely used in Iranian traditional
medicine as a gastro-protective and wound healer agent. In the present study the hepatoprotective
property of T. graminifolius DC. aqueous extract (TGAE) was investigated against carbon
tetrachloride (CCl4) induced hepatocellular injury in mice. Methods: Fifty male mice were divided
into five groups (n=10). Group 1 (control) received 1 ml/kg olive oil intraperitoneally (i.p.) and
distilled water orally; Group 2 (untreated) received CCl4 (50% in olive oil, 1 ml/kg; i.p.); Groups 3, 4
and 5 received CCl4 and 30, 90 and 270 mg/kg of TGAE (TGAE30, TGAE90 and TGAE270),
respectively. At the end of the 45-day treatment, the mice of all groups were euthanized, then blood
and liver samples were collected for biochemical and histological parameters analysis. The data was
analyzed by one way variance analysis and Duncan’s test using SPSS 21. Results: Different doses of
TGAE (especially TGAE270) could significantly (p≤0.05) reduce the raised levels of ALP, AST, ALT,
Cholesterol, LDL and increased HDL, SOD, CAT as compared to the untreated group. The weight
and volume of the hepatic structures decreased significantly (p≤0.05) in several doses of TGAE
(especially TGAE270) when compared to the untreated group. Conclusion: The results of the present
study showed that under the present experimental conditions, TGAE indicated hepatoprotective
abilities against CCl4 induced liver damage in mice.
Keywords :
aqueous extract , CCl4 , hepatoprotective activity , Tragopogon graminifolius
Journal title :
Research Journal of Pharmacognosy